Optimizing Biobutanol Production: Analysis of Feedstock Selection, Pre-treatment Methods, and Microorganism Strains for Biobutanol Production from Agricultural and Industrial By-products

Kriss Spalvins; Zane Kusnere; Svetlana Raita; Indra Berzina

doi:10.7250/CONECT.2023.058

Optimizing Biobutanol Production: Analysis of Feedstock Selection, Pre-treatment Methods, and Microorganism Strains for Biobutanol Production from Agricultural and Industrial By-products

Authors

Kriss Spalvins Riga Technical University, Institute of Energy Systems and Environment
Zane Kusnere Riga Technical University
Svetlana Raita Riga Technical University
Indra Berzina Riga Technical University

DOI:

https://doi.org/10.7250/CONECT.2023.058

Keywords:

Acetone-butanol-ethanol (ABE) fermentation, agricultural by-products, biobutanol, bioprocess optimization, chemical hydrolysis, feedstock, industrial byproducts, microbial hydrolysis, pretreatment methods

Abstract

This study presents an analysis of key elements of biobutanol production, including feedstock selection, pre-treatment methods, and microorganism strains. Using laboratory experiments, we evaluated the ability of different microorganisms to convert various agricultural and industrial by-products into biobutanol. We tested three microorganism strains: C. acetobutylicum DSM 792, C. beijerinckii DSM 6423, and C. saccharoperbutylacetonicum DSM 14923. The results showed that biodiesel production residues, various agricultural hydrolysates, yeast residues, and milk processing residues were the most suitable feedstocks for biobutanol production. In addition, the authors explored different pre-treatment methods, such as microbial and chemical hydrolysis, to enhance the efficiency of biobutanol production. Our findings provide valuable insights for optimizing biobutanol production processes in line with sustainable and cost-effective production principles.